FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56167126
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA906550.09.65310894-95
GATCGGA990150.09.57524894-95
AGATCGG1153200.09.57122894-95
TAGATCG350450.09.301537594-95
ACACGTC315650.08.71083494-95
TCGGAAG896150.08.23540994-95
CGTCTGA230300.08.16766294-95
CACACGT408600.07.932968694-95
AAGATCG398050.07.55828194-95
CGGAAGA920650.07.518965294-95
ACGTCTG297400.07.468433494-95
CAGATCG441200.07.06811494-95
GCACACG500600.06.987202694-95
GAGATCG453150.06.627697594-95
CACGTCT341950.06.60253994-95
AGAGCAC809550.06.375968594-95
AGCACAC697750.06.14153494-95
GAAGAGC1205200.05.639528894-95
GAGCACA830350.05.434889394-95
CTAGATC240000.05.166898394-95
Produced by FastQC (version 0.11.5)