FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54908732
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA318950.010.68687494-95
GATCGGA1540700.010.135303594-95
ATCGGAA1387800.010.02290294-95
ACACGTC442950.09.93777194-95
TAGATCG501500.09.27134494-95
AGATCGG1727550.09.18887294-95
ACGTCTG390850.09.12672994-95
CACACGT554500.09.07766794-95
CGGAAGA1324750.08.45623594-95
CACGTCT462850.08.38030294-95
GCACACG679050.08.34282794-95
AAGATCG561200.08.27019294-95
TCGGAAG1304050.08.0024992-93
CAGATCG602450.07.639267494-95
GAGATCG656900.07.548238894-95
AGCACAC879950.07.42783594-95
GAGCACA1036400.07.00729494-95
AGAGCAC1042900.06.99296894-95
AAGAGCA1267450.06.62560694-95
GAAGAGC1501450.06.347269594-95
Produced by FastQC (version 0.11.5)