FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54493603
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA361750.010.58668994-95
ACACGTC531800.010.1966394-95
ACGTCTG446400.010.01641394-95
CACGTCT525450.09.26853994-95
CACACGT642600.09.23645494-95
ATCGGAA1658050.08.89101994-95
GATCGGA1817550.08.63278494-95
TAGATCG613850.08.4220894-95
AGATCGG2048550.08.38729194-95
CGGAAGA1542850.08.32660694-95
TCGGAAG1585100.08.31720594-95
GCACACG767950.08.2094594-95
AGAGCAC1206000.07.756108894-95
AAGATCG646700.07.36606694-95
AGCACAC997100.07.25443194-95
CAGATCG670550.07.10407194-95
GAGCACA1169250.06.98718994-95
GTCTGAA519350.06.820796594-95
AAGAGCA1457850.06.795130394-95
GAAGAGC1682050.06.622823794-95
Produced by FastQC (version 0.11.5)