FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005208003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005208003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46526974
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1037950.09.44478994-95
GATCGGA899350.09.31676194-95
ATCGGAA818800.09.26218794-95
TAGATCG304200.09.05915194-95
ACACGTC290000.08.95811394-95
CGTCTGA212550.08.263514594-95
TCGGAAG810550.08.09661494-95
CACACGT368650.08.08107894-95
ACGTCTG267550.07.94899694-95
AAGATCG347100.07.78257494-95
CGGAAGA819950.07.555447694-95
GCACACG454150.07.48309894-95
CACGTCT309050.07.383826394-95
CAGATCG390050.07.21882494-95
GAGATCG413450.06.566566594-95
AGCACAC606600.06.419479494-95
AGAGCAC716650.06.391239694-95
GAAGAGC1057250.05.581454894-95
CTAGATC197750.05.32918394-95
GAGCACA716400.05.085887494-95
Produced by FastQC (version 0.11.5)