FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005208009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005208009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59297345
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1049250.010.205523594-95
AGATCGG1206300.09.864994-95
TAGATCG378250.09.65893994-95
ATCGGAA956100.09.57417594-95
ACACGTC329150.08.86514794-95
TCGGAAG942200.08.49600694-95
CGTCTGA238550.08.32726994-95
CACACGT420700.08.04623894-95
AAGATCG423200.07.94161794-95
ACGTCTG300450.07.56765794-95
CACGTCT361050.07.52425494-95
CGGAAGA944200.07.42607794-95
CAGATCG442100.07.30458294-95
GCACACG499700.07.187817694-95
GAGATCG469500.06.96976994-95
AGAGCAC834350.06.37040794-95
AGCACAC728200.05.87976994-95
GAAGAGC1248950.05.648466694-95
TTAGATC230450.05.19527394-95
GAGCACA846600.05.18112594-95
Produced by FastQC (version 0.11.5)