FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005208023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005208023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54101002
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA940400.08.81133694-95
GATCGGA1023400.08.78003494-95
CGTCTGA248050.08.71485194-95
AGATCGG1163850.08.572227594-95
ACACGTC342600.08.35808694-95
TAGATCG347200.08.34629194-95
TCGGAAG929900.07.879104694-95
ACGTCTG310050.07.73189994-95
CACACGT429850.07.546365394-95
CGGAAGA940250.07.46877994-95
CACGTCT361800.07.24312594-95
GCACACG517450.06.823633794-95
AAGATCG398200.06.735071794-95
CAGATCG421050.06.68425594-95
GAGATCG481100.06.314058394-95
AGAGCAC834250.06.14420394-95
AGCACAC713250.05.837990394-95
AAGAGCA1052050.05.63253794-95
GAAGAGC1217750.05.45045194-95
GAGCACA841750.05.37819594-95
Produced by FastQC (version 0.11.5)