FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005208027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005208027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61985728
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA366050.010.75646494-95
ACACGTC520100.010.49937994-95
ACGTCTG439700.010.15116394-95
CACGTCT516500.09.47906194-95
ATCGGAA1653400.09.33234794-95
AGATCGG2051750.09.19883994-95
CACACGT629450.09.17274394-95
TAGATCG626250.08.99801394-95
GATCGGA1799000.08.9304694-95
GCACACG743950.08.56354394-95
AAGATCG661650.08.04832994-95
CGGAAGA1525450.07.98884794-95
TCGGAAG1558900.07.89195894-95
AGAGCAC1222800.07.75964894-95
AGCACAC1013400.07.75157294-95
CAGATCG665650.07.646027694-95
GAGATCG704800.06.93740294-95
GAGCACA1180950.06.834902394-95
GAAGAGC1736350.06.74899494-95
GTCTGAA604450.06.33782894-95
Produced by FastQC (version 0.11.5)