FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005208385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005208385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162706
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3751.769331E-89.46605140-144
GTCGCCG4005.0640665E-99.396447140-144
TCGCCGT4004.604445E-57.862879140-144
CGGCGTG3500.00360684756.5625978140-144
GCCGTAT5252.77274E-56.3637314140-144
GGTCGCC5658.074867E-55.9132013140-144
GTGGTCG7201.44312635E-55.5102625140-144
TCGGTGG9104.9977407E-65.048152140-144
Produced by FastQC (version 0.11.5)