FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005212211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005212211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22865122
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG188900.014.7201112
ATGGGGG239550.012.18355751
TGGGGGG440250.010.9004982
TAGGGGG97700.09.9575961
AAGGGGG209350.08.8779211
ACGATCG15350.08.029139145
TCGCGTA10250.07.63885640-44
TATGCCG46750.07.536731745-49
CGCGTAT11150.07.412392640-44
GATCGGA235200.07.1922191
ATCGGAA239000.07.1082422
TCGGAAG240300.06.9996753
CGGAAGA247600.06.734744
CGTATGC54850.06.291566445-49
ATGCCGT59200.06.04969845-49
CTCGTAT48000.05.5884185145
TGCCGTC65350.05.546931750-54
ATCGCGT13900.05.52865540-44
GCCGTCT66550.05.490487650-54
TAGATCG79550.05.285867145
Produced by FastQC (version 0.11.5)