FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005212313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005212313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21361898
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233740.10941911622272515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG184700.020.8766692
ATGGGGG239650.016.3322091
TGGGGGG463900.015.5751512
AAGGGGG202700.012.9684591
TAGGGGG97000.011.7533441
TCGCGTA8105.010024E-48.95038145
TTGGGGG292300.07.7013421
ACGGGGG57000.07.26162051
TCGGGGG70500.07.10711771
CGTCTGA154400.06.010204145
ATTGGGG134700.05.7144151
GATCGGA238450.05.685528145
ACACGTC170850.05.5163884145
ATCGGAA237200.05.104207145
CGGGGGG178350.05.08944562
AGATCGG249300.05.0600343145
CTCGTAT43307.1668182E-95.022961145
CGTATGC44753.1432137E-95.0222135145
Produced by FastQC (version 0.11.5)