FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005212411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005212411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23760752
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374790.1577349067066564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG243150.023.2009812
ATGGGGG279300.021.0558931
TGGGGGG645750.016.5277042
AAGGGGG236500.016.3013271
TAGGGGG108500.014.6545991
TTGGGGG353550.09.1794411
ATTGGGG144600.08.5859311
ACGGGGG76950.06.6046371
GATCGGA237400.05.6190853145
TAGATCG87550.05.3825207145
AATGGGG260750.05.2068721
AGATCGG252500.05.168204145
ACTCGAC80900.05.108047145
CTCGACA86300.05.0404463145
Produced by FastQC (version 0.11.5)