FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005212413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005212413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23703771
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475580.20063474288542527No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322030.13585602054626666No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289590.12217043440050107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG269800.028.906332
ATGGGGG304450.024.9247591
AAGGGGG246700.020.9281641
TAGGGGG124500.019.0725631
TGGGGGG783650.018.4492952
TTGGGGG381400.011.8804951
ACGGGGG81900.010.0190181
ATTGGGG152850.08.5989171
CTGGGGG410300.07.1146771
TCGGGGG96750.06.52978561
AATGGGG254300.06.05368951
CGGGGGG282800.06.0085142
TATGGGG143900.05.55088851
CTCGTAT50650.05.439157145
ACTCGAC84450.05.236699145
ACACGTC183700.05.0910573145
Produced by FastQC (version 0.11.5)