FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005212869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005212869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23713276
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286300.1207340563151207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG216150.020.694512
ATGGGGG274600.017.6646731
TGGGGGG563600.015.9120312
AAGGGGG226300.013.1561541
TAGGGGG110000.011.88254451
TTGGGGG332350.08.5867051
ACGGGGG64050.06.12215471
ATTGGGG157000.06.0590051
TCGGGGG79050.05.60347751
AGATCGG202150.05.271938145
CGGAAGA208400.05.2181945145
ACACGTC143250.05.2127795145
CTGGGGG363350.05.17611931
GATCGGA194300.05.111808145
ATCGGAA199550.05.0863123145
Produced by FastQC (version 0.11.5)