FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005213071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005213071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19287256
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG457720.2373173249735473No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297120.15404990735851695No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257870.133699682318729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG238150.030.8496442
ATGGGGG254750.026.5881231
TAGGGGG95900.023.6187131
AAGGGGG203250.023.1811181
TGGGGGG672100.018.9135272
TTGGGGG308800.012.6245881
ATTGGGG125200.012.1768581
ACGGGGG63950.08.7411871
CTGGGGG319700.07.62986561
TCGGGGG82800.07.5402961
TATGGGG116750.06.7156361
ATAGGGG70800.06.4599261
TCTCGTA53450.05.968068145
ACACGTC201250.05.6197557145
AATGGGG208450.05.57234951
ATCGGGG29059.854302E-75.49789951
TATGCCG44358.185452E-115.3944736145
ATCGTCT90450.05.290103145
TAGATCG96350.05.2671432145
CGGGGGG238500.05.2355262
Produced by FastQC (version 0.11.5)