FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005213073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005213073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19409833
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG585470.3016357739914609No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405140.20872925593950242No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353490.18211903214211064No Hit
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213210.10984638559229233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG274550.034.830652
ATGGGGG274750.032.1096731
AAGGGGG220000.028.284951
TAGGGGG111800.025.0693341
TGGGGGG812850.021.5101452
TTGGGGG341400.016.9508721
ATTGGGG130300.014.3214181
ACGGGGG66150.011.7449511
CTGGGGG324800.09.9928361
TCGGGGG84900.09.7497711
AATGGGG216500.08.4516191
TATGGGG117650.08.2083711
ATAGGGG79550.07.5759171
ATCGGGG27702.5465852E-117.07752281
CGGGGGG271750.06.70658542
TCGGAAG293500.06.0024137145
CGGAAGA292100.05.4851494145
CACATCG108850.05.461516145
ATCGGAA290150.05.2721486145
CTCATCG38651.3014869E-85.25214145
Produced by FastQC (version 0.11.5)