FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005213153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005213153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41412379
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425880.10283881541796958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG352800.019.8399182
ATGGGGG445050.015.1401661
TGGGGGG889250.014.0278372
TAGGGGG183500.012.226831
AAGGGGG381150.012.0205661
CGATCGA100100.06.880455145
TTGGGGG562750.06.79964971
CCGATCG107700.06.664186145
ACCGATC132400.06.3518124145
ACACGTC324200.05.8365355145
ATTGGGG263900.05.80543141
CGATATC103500.05.743824145
GATCGGA460500.05.699104145
TAGATCG165650.05.4707417145
CGGAAGA472250.05.388437145
TCACCGA192700.05.379996145
CGTCTGA294350.05.3693304145
AGATCGG490800.05.2438645145
ATCGATA116350.05.109461145
CACCGAT150700.05.099414145
Produced by FastQC (version 0.11.5)