FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005213154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005213154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41412379
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG146450.06.83157145
TCGATCG97700.06.8269086145
AGCGTCG311200.06.0105047145
ATCGATC123100.05.9483175145
CGTCGTG283350.05.910437145
GTCGTGT294100.05.817652145
GCGTCGT289700.05.7558584145
CGATCGG96200.05.7275558145
GATCGGA457500.05.6572833145
AGAGCGT385400.05.4740915145
CGTGTAG274750.05.462148145
TCGTGTA277650.05.4573197145
GATCGGT108500.05.4123526145
TATCGAT138000.05.4111423145
GAGCGTC362200.05.344334145
CGGAAGA471200.05.2773952145
ATCGGTG118200.05.2748704145
AGATCGG491100.05.2406993145
GTATCGA130750.05.212151145
ATCGGAC23952.4940603E-45.1460524145
Produced by FastQC (version 0.11.5)