FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11189424
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC91050.012.9669468
GAGCACA95550.012.35625659
GATCGGA116750.010.5793411
TCGGAAG133600.09.1065943
ATCGGAA156250.08.2012042
AAGAGCA155650.08.0015247
GAAGAGC201100.06.6585036
GGAAGAG203700.06.43206265
GTGGCAT84550.06.0453099
ACACACA43300.06.0189335-39
GTATACA35101.9281288E-106.00284961
GTATACG41751.8189894E-125.9168291
CACACAT45200.05.606642235-39
CGGAAGA248400.05.5934724
GACACAC50650.05.401355735-39
CAGTCAC50150.05.34100625-29
CAACGGT99250.05.29502534
AACGGTG102850.05.2496085
GTGTATT57551.8189894E-125.04988241
Produced by FastQC (version 0.11.5)