FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773457
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG9050.019.883324
TCTAGGC9200.019.5586685
TCTCTAG14000.012.8531463
CTAGGCA13200.012.5412566
GTATGCC200350.06.3231352
GTCTTCT169650.06.3214919
GCCGTCT176800.06.18797356
GGTTAAA90550.06.02513931
CGTCTTC195150.05.86429368
GGTACTA51100.05.8365641
GGTCTTA60050.05.6934981
GGTACGA86300.05.56323581
GGTTAGC31451.3179306E-75.5511751
GGTATGA149350.05.50386241
CTTGCGT103650.05.34725052
GGTATAC76350.05.3354781
GGTATCA193850.05.21606871
GGTCTAA27951.570731E-55.20526081
GGTCCAA36509.920586E-85.18173031
GGTACAA122650.05.1599561
Produced by FastQC (version 0.11.5)