FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773457
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC11201.364242E-1011.567575
CTCTAGG9904.1085877E-710.182344
CTAGGCA13453.3789547E-89.0972296
TCTCTAG14501.0189106E-67.94511563
GGTCTAA29200.07.7810341
GTTGAGT63600.06.9032347
GGTATAA146100.05.86940531
CGTTGAG87450.05.59669456
TGAGTTG91000.05.5364669
GGTTCGA96100.05.49120431
GGTTCAC97800.05.47069451
GGTATTA137450.05.4389351
GGTAACG112150.05.42421961
GGTACAA120400.05.17429161
GGTTAAA91800.05.10970451
GGTACTA56001.8189894E-125.10428671
Produced by FastQC (version 0.11.5)