FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13175606
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA56100.034.6505975
CAGTGGT59500.032.0735864
GTGGTAT75300.025.4329366
AGCAGTG89850.021.7223152
GGTATCA100500.019.986948
TGGTATC102450.019.606597
GCAGTGG101350.018.9003543
GTATCAA115050.017.5218359
AAGCAGT141900.014.3085321
GAGCTAA75600.010.9518095
GTGTGTC50650.09.4489581
GTATTGA161100.08.9122851
TGAGCTA98500.08.77331354
GTATATT120000.08.5807751
TTAGTGG21455.456968E-128.3931453
GTCTCTA45600.08.2690941
CTAAAAG103800.08.2538738
AGCTAAA106950.08.1454386
GTATAGA62650.07.8705891
TAGTGGG26301.8189894E-127.66691974
Produced by FastQC (version 0.11.5)