FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922121
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA94150.022.9633836
ATGGCTC100150.021.3715175
CTCAAAC107100.021.2635569
GGCTCAA105400.020.9227667
TCTGATG116100.017.9256951
GATGGCT123950.016.7445764
GCTCAAA158500.014.2770568
CCTCTAG11850.012.63675650-54
CTGATGG190200.011.9991772
CTCTAGG7450.011.01638650-54
TGATGGC215350.010.9634173
CTAGCAC8050.09.12210250-54
TCTAGGA10000.08.35119450-54
CTCGGGT55350.07.77680520-24
CTAGGAT10300.07.40899450-54
GGGTGGG65250.06.883664625-29
CTTGGGA62900.06.29119251
TCGGGTG76550.06.036781325-29
AACACAG83500.06.0355710-14
CTCTAGC12250.05.99452350-54
Produced by FastQC (version 0.11.5)