FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14309042
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA122550.031.5809087
CTCAAAC123700.031.520369
ATGGCTC127300.030.3449445
TGGCTCA133000.029.6412336
TCTGATG143350.026.5840781
GCTCAAA167500.023.6222448
GATGGCT165900.023.1976954
CTGATGG244800.015.8507172
CCTCTAG17600.013.82545950-54
CTCTAGG11450.012.57476950-54
TGATGGC340450.011.819353
CTCGGGT64150.011.78183520-24
CTAGGAT14250.010.40705650-54
TCTAGGA14050.010.35024450-54
AACACAG92550.09.13285110-14
CTAGCAC11550.08.97544650-54
CTCTAGC14450.07.771990350-54
TCGGGTG101750.07.69691325-29
ACACAGC115250.07.546415310-14
AAACACA114750.07.47890910-14
Produced by FastQC (version 0.11.5)