FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12635879
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC5650.020.382165
CTCTAGG6150.019.8957064
CTAGGCA7908.0035534E-1114.5771146
TCTCTAG10304.1836756E-1011.8794753
TTGGAGT57100.08.5713948
GTCTTCT86150.08.4381029
GTCTAAT21051.6407284E-97.60165551
TGGAGTG60250.07.2870459
GTCCTAA16755.657001E-56.5134951
CGTCTTC136200.06.1299878
GTCTGAG39953.6379788E-126.0080671
GTCCAAA66050.05.94646071
CGTTGGA80950.05.86821136
CTTGCGT83650.05.59293562
GTCAGAG60050.05.57163241
GTATGCC137800.05.48444272
CCGTCTT145800.05.43017537
GTCTTAT50153.6379788E-125.36621951
GTTGGAG93600.05.3058137
GTCTAAA26052.7191754E-55.3049771
Produced by FastQC (version 0.11.5)