FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12635879
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG7204.9058144E-914.0016754
TCTAGGC8503.6925485E-912.7020225
CTAGGCA9401.2569217E-912.2515476
TGAGTTG46250.010.8938139
GTTGAGT44750.09.9722297
TCTCTAG10851.438453E-58.6275893
GTCTAAG13151.5102531E-57.8013551
GTCTAAA26706.548362E-117.1355891
GTCTCAA54050.06.64305931
GTCTGAG41400.06.37191441
GTCTAAT21858.294908E-66.036551
TTGAGTT89000.05.8228578
TGTGCGT91600.05.34710032
GTCCAAA64950.05.18974971
TGCGTTG98950.05.1668644
Produced by FastQC (version 0.11.5)