FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11128382
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTTCAGGTGTAGATCT144020.12941683705681564Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT162250.014.5099358
GATCGGA174400.014.1763431
GAGCGTC188950.012.650099
AAGAGCG191100.012.6204517
TCGGAAG194600.012.6140773
ATCGGAA227250.011.4698542
GAAGAGC223400.010.7312876
GGAAGAG253150.09.9250885
TAGATCT53250.08.89716640-44
CGGAAGA303000.08.3629144
AGTGTAG59700.08.03153325-29
GAGTGTA65350.07.40324725-29
GTAGATC74500.06.881278540-44
AGAGTGT77850.06.381004325-29
GTAGCTT79650.06.34490230-34
GTGTAGA82200.06.341794540-44
TAGCTTC80000.06.191159730-34
AGATCTC83050.06.10285145-49
TGTAGCT80000.05.957191530-34
AGGTGTA88250.05.82459435-39
Produced by FastQC (version 0.11.5)