FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13254952
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC135250.10203733668745084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA123100.034.833317
CTCAAAC134450.031.8927489
ATGGCTC138900.030.7667565
TGGCTCA143650.029.7996886
GCTCAAA163900.026.6018758
TCTGATG170200.025.3086011
GATGGCT178850.024.2165074
CTGATGG226950.019.2870852
TGATGGC279050.016.1997593
CCTCTAG19850.013.92699250-54
CTCGGGT69550.012.04674520-24
AACACAG100350.09.3841810-14
GGTCCTC92100.09.09849345-49
CTCTAGG18400.08.76429150-54
GGTGGGA100250.08.64493225-29
TCGGGTG99200.08.591310525-29
ACACAGC106450.08.5623710-14
TGGGAAC103950.08.44869930-34
TCTAGGA18500.08.40559950-54
CTAGCAC15600.08.39911350-54
Produced by FastQC (version 0.11.5)