FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15103690
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC7356.5974746E-913.7096175
CTCTAGG6853.8711732E-813.6599874
CTAGGCA8858.805E-811.3859526
TCTCTAG8909.5154974E-811.3223233
GTCTTCT125650.09.2797679
TGGAGTG76050.08.5178089
TTGGAGT85100.07.78113278
CGTCTTC164550.07.2172328
CTTGCGT88500.06.9945752
GTATGCC161150.06.8785452
CGTTGGA104100.06.4300866
CGTATGC127750.06.3766321
CCGTCTT194350.06.14763557
CTAGATC12000.05.8811827140-144
GCCGTCT216200.05.59291466
TATGCCG240150.05.4249223
GTTGGAG125600.05.38677
TAGGACC17550.00513076275.3316763
TGCGTTG113700.05.31760844
GCGTTGG142450.05.0526845
Produced by FastQC (version 0.11.5)