FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14521765
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATCGCTGTGTAGATCT271900.18723619339660158Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT359150.012.7399968
GATCGGA381850.011.8558551
GAGCGTC386300.011.8418179
AAGAGCG389750.011.6841167
TCGGAAG392500.011.6399933
GAAGAGC438050.010.4948176
ATCGGAA444000.010.4913192
GGAAGAG480700.09.6988685
CGGAAGA528300.08.9099874
TGTGTAG110250.08.34582240-44
GTGTCTA115700.08.12471125-29
TAGATCT120100.08.05699540-44
CGTATCA143450.07.084685360-64
AGTGTCT138500.07.015878725-29
CTGTGTA137450.06.954133535-39
TCGCTAG12500.06.9103845-49
GAGTGTC140950.06.802009625-29
TCTATCG145250.06.798732830-34
ACACTAG11650.06.795889115-119
GTAGATC149000.06.735864640-44
Produced by FastQC (version 0.11.5)