FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14268217
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA127450.020.188117
CTCAAAC140500.017.8513159
ATGGCTC168800.015.4557355
TGGCTCA175400.014.9566566
GCTCAAA180100.014.5264738
TCTGATG220550.011.5925561
GATGGCT240450.010.8202174
CTGATGG269050.09.7127992
CTCGGGT59200.08.68139320-24
TGATGGC325200.08.4554143
GGGTGGG62800.08.04611325-29
CCTCTAG20950.07.491379350-54
GGTCCTC73800.07.15994845-49
AGGTCCT84300.06.13150445-49
GGTGGGA87000.05.923827625-29
ACACAGC94650.05.88759210-14
CAGGTCC93500.05.805369445-49
AACACGT58000.05.7835830-34
AACACAG99350.05.73950710-14
CCTCGGG97300.05.711068620-24
Produced by FastQC (version 0.11.5)