FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19828607
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC21150.010.3053971
GTCTAGA5700.002515892510.196921
GTCTAAA37900.08.6263511
GTCTGAG62600.07.54384761
GTCCAAA94200.06.9413751
GTCCAAG82700.06.67670151
GTCTGGA163650.06.34854031
GTCTAAG24402.2281893E-76.25292731
GTCAAAA243000.06.06937031
GTCTTAT112700.06.05979351
GTCTTAG32751.4315447E-95.98971751
GTCAAAG233200.05.88826231
GTCTCTA33352.1991582E-95.8819561
GTCACAA97150.05.83318421
GTCACGA112700.05.801931
GTCTATA75150.05.7039651
GTCACAG101550.05.6519851
GTCTAAT33701.70985E-85.60528041
GTCTTGA164350.05.5699941
GTCACAC68250.05.53547051
Produced by FastQC (version 0.11.5)