FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13359235
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG7201.0408476E-612.0008394
TCTCTAG8455.9213016E-711.0775313
CTAGGCA9854.2477095E-69.4996456
TCTAGGC8508.487461E-59.3148795
GTTGAGT50750.07.8005517
TGAGTTG60100.06.70675049
GTGCGTT81500.05.3892533
CCTAATC21650.00165933215.07722141
Produced by FastQC (version 0.11.5)