FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16846912
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC73500.010.7770541
GTATATT208300.08.9537551
CCTTCAT275550.08.7546571
GTATACC56850.08.6133721
GTATAGA102450.08.5751671
GTATATC162400.07.8484161
GTATAGG57700.07.73767661
CCTACAT92650.07.6945731
TGTGTCA105050.07.60860252
GTATATG133450.07.60843371
TAATACG68800.07.43006238
TAAGGGG38700.07.25657372
CCTTCAC227700.07.1788941
GTATAGC83350.07.08439871
CCTGCAT202800.07.0661011
GTATATA126250.06.9586211
TGAGGTA103600.06.9505353
CATTACC147400.06.88836861
CCTTGAT295700.06.86740261
CATGCAT64750.06.78398371
Produced by FastQC (version 0.11.5)