FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14134371
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC386240.2732629559532575Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGTATACCACTATAGACAATTAGCGACAATTCCTTTCCCACTTTATCA373940.26456076467781975No Hit
GGGTATACCACTATAGACAATTAGCGACAATTCCTTTCCCACTTTATCAT157650.1115366223229884No Hit
GGGGAGTATGTAATAATATACCCCCACGCCGCCATTTCCCCATTCCGGTT153800.10881276570425384No Hit
GGGAGTATGTAATAATATACCCCCACGCCGCCATTTCCCCATTCCGGTTG149510.10577761118623533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCC85200.070.493561
GGGGGAC115800.066.773991
GATCGGA158300.059.3942072
GGGGAGC115500.058.6575051
GGGGACC89400.058.5000761
AGATCGG162100.057.680441
GGGGACG113850.056.6405451
GGGGGGC93700.055.970271
GGGGGCT62900.055.3540341
GGGGTAT168800.054.4456061
GGGGAGT95650.053.464171
GGGGCCG93250.050.484091
GGGGACT71950.050.004551
GGGGGGA111700.050.003021
GGGGGAT119850.049.507841
GGGGATC77500.049.418651
GGGGGTC58350.049.103781
TCGGAAG187250.049.0688364
GGGCCGG100700.047.829661
GGGGGCG122900.046.449551
Produced by FastQC (version 0.11.5)