FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14879611
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC665500.4472563160421331Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC177400.073.513331
GGGGGCC167800.072.791411
GGGGACG178650.057.3273241
GATCGGA290700.056.3755152
GGGGGCA124050.054.903351
GGGGGGC185300.053.9782561
AGATCGG300750.053.9257471
GGGGACC153900.053.680321
GGGGGCT114150.053.437991
GGGGGGA182300.051.4448471
GGGGAGC194450.051.0279541
AGAGCGT319150.050.5154469
TCGGAAG329600.049.1774
GGGGGAT192750.048.1289251
GGGGGAG189500.047.8060871
GGGGATC130750.047.4856151
GGGCGGG213500.047.2902221
GGGGAGA266900.046.7966271
AAGAGCG355500.045.8392458
GGGGCGA196250.045.6074181
Produced by FastQC (version 0.11.5)