FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17781998
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG646060.3633225017796088TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202750.11401980812279924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA366500.055.589042
AGAGCAC356350.055.5450179
AGATCGG388300.052.7313351
TCGGAAG407450.049.3443264
GGAAGAG429000.046.680496
GATCTAG19850.046.3876578
ATCGGAA455900.044.48143
AAGAGCA450950.044.2626158
GAAGAGC467550.042.644587
CCGATCT169500.041.4926386
CGGAAGA488700.041.0815855
TCCGATC191700.036.762985
CGATCTG168100.033.9442527
GATCTGG157650.030.5374898
CGATCTA45200.029.5147177
CTTCCGA251850.028.7881413
ATCTAGG24000.027.4909279
ACACGAC69500.026.9147573
TTCCGAT284900.025.2198724
ATCTGGG131650.024.8379049
Produced by FastQC (version 0.11.5)