FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17781998
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC601130.3380553748796957Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG378950.047.720191
GATCGGA381350.047.5006982
AGAGCGT383200.046.5162439
AAGAGCG415800.043.4443058
TCGGAAG444300.040.4129034
ATCGGAA497750.036.104483
GGGATCA118950.035.6131321
GGGGACT94800.034.508811
GCGACTA76250.030.8843579
GAAGAGC636000.028.8128877
GGGGACG159950.027.9809651
GGGGATC125300.027.4403131
CGGAAGA680450.026.8134165
GGGATGT193050.026.4899481
GGGGTAT136900.025.6980441
GGGACTA70250.025.6074961
GGATCAA115700.025.3734282
GGGGTTT121550.025.3627151
GGGTAGA128350.025.3188441
GGGCCGA117500.024.817041
Produced by FastQC (version 0.11.5)