FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14777841
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA2089061.41364357621658TruSeq Adapter, Index 7 (97% over 38bp)
CCTCCGCAACTAATTCCTCTAATTCACATCATTTTAATCTCTTAAATTCT236230.15985420333051356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC72350.083.3808755
CGATCTG59400.080.8055957
GATCTGG47250.080.714928
CCGATCT82050.073.347296
GATCGGA597650.073.129612
AGATCGG602250.072.6685941
TCGGAAG664000.064.871954
AGAGCAC669650.063.739729
GAAGAGC674250.063.3809977
GATCTAG12900.061.8262448
CGGAAGA708050.060.817855
ATCGGAA717450.060.6045153
GGAAGAG713550.060.1564066
CCTCCGC70150.056.4337961
CGATCTA21400.053.193167
CGCAACT76000.052.472385
TCCGCAA82200.048.4242673
AAGAGCA899200.047.8154538
CTCCGCA83950.047.5016752
CTTCCGA128200.047.393473
Produced by FastQC (version 0.11.5)