FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14777841
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC472520.31974900799108613Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATA413930.28010180918850053Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTATATA153870.10412211093623217Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC209650.083.1335751
GGGGACG162200.077.4487841
GGGGGCA112400.075.627351
GGGGGCC236100.072.038461
AGAGCGT562200.070.970889
GGGGGCT94850.068.7448041
GGGGAGA199100.068.304221
GATCGGA596950.067.508792
AGATCGG598600.067.22291
AAGAGCG597000.067.149428
GGGGGAA143650.066.395361
GGGAGGA158650.065.832441
GGGAGGC138250.065.21141
CCAGTCC31050.064.704691
GAAGAGC637750.062.926977
TCGGAAG640400.062.7572944
GGGGAGG141650.062.468461
GGGGCGA169750.061.6558231
GGGGGAT160500.060.3286741
GGAGGGC119550.059.880461
Produced by FastQC (version 0.11.5)