FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12213037
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1970081.6130959072669642TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA511500.076.13242
AGAGCAC504300.075.221259
AGATCGG523200.074.5284351
TCGGAAG531150.072.4974444
GAAGAGC542550.070.2657
GGAAGAG545150.070.184886
AAGAGCA568600.066.969368
CGGAAGA583400.066.068255
ATCGGAA595100.065.144073
GATCTAG16750.042.852748
TCCGATC132650.037.441875
CCGATCT138250.035.9775356
CTTCCGA158800.031.5944163
ATCTAGG17000.031.1340589
CGATCTA30050.028.2292677
CGATCTG148450.027.3017087
ATCTGGG101700.025.2373719
GATCTGG141550.024.6885418
TTCCGAT205500.024.4856264
TCTTCCG302850.017.1174262
Produced by FastQC (version 0.11.5)