FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16020715
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA2234111.3945132910734632TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA737250.065.516282
AGAGCAC729250.064.5350959
AGATCGG754100.064.245851
TCGGAAG778200.061.053384
GAAGAGC784200.060.1979377
GGAAGAG798950.059.214746
AAGAGCA829100.057.0515948
ATCGGAA852450.056.2534643
CGGAAGA853500.055.6253435
GATCTAG22050.052.9392858
TCCGATC158250.045.2669035
CCGATCT175050.040.922656
CGATCTA38850.038.4447447
ATCTAGG19700.036.435929
CTTCCGA202150.036.2244383
GATCTGG149650.030.5228188
CGATCTG192400.029.6195357
ATCTGGG121100.028.6183479
TTCCGAT268800.027.2968184
CTCTTCC306650.025.4884451
Produced by FastQC (version 0.11.5)