FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10524572
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG288890.2744909721744504TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG153350.055.9331321
GATCGGA153600.055.6516882
AGAGCAC154800.053.25349
TCGGAAG167550.050.326084
GAAGAGC188350.044.2299427
GGAAGAG193400.043.3009836
CGGAAGA197000.043.024565
ATCGGAA204400.041.64273
AAGAGCA238150.035.0112158
TCCGATC77050.033.1236465
CCGATCT83650.030.2504446
GATCTAG14400.028.6993628
CTTCCGA96900.027.5344033
GATCTGG70550.023.6369118
CGATCTG88900.022.5096767
TACACGA43200.021.314532
TTCCGAT127750.020.6017254
CGATCTA29200.018.37437
ACACGAC50200.017.9089283
ATCTGGG76800.016.8985589
Produced by FastQC (version 0.11.5)