FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10524572
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC322160.30610270897476877Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC141150.073.8516851
GGGGGCC145850.070.0791851
GGGGGGC134100.064.372841
GGGGGGA142500.061.443641
GGGGGTC78950.060.8261531
GGGGGCT80300.058.448491
GGGGGCA102850.055.1552661
GGGGACC130350.052.6459051
GGGGCGA127300.052.3686831
GGGCGGG153650.050.611211
GGGGGCG155800.050.5180781
GGGGGAA147950.049.3250541
GGGGACG157700.049.0354351
GGGGGAG156350.048.2989121
GGGGAGC178600.048.0087661
GGGGAAC108600.045.6889081
AGATCGG176000.045.049711
GGGGAGA221800.044.6759381
GGGAGGC125700.044.2056731
GGGGAGG173300.044.119091
Produced by FastQC (version 0.11.5)