FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11904415
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1290721.08423639464854TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG359250.070.7380751
GATCGGA360500.070.411172
AGAGCAC354650.069.8354959
TCGGAAG370850.067.350684
GAAGAGC385800.064.3850867
GGAAGAG396800.062.9476556
ATCGGAA412950.060.8173983
AAGAGCA411400.060.3610088
CGGAAGA420150.059.7079165
GATCTAG10800.028.1958838
CCGATCT119050.025.1525866
TCCGATC126400.023.7472555
CTTCCGA138400.022.5257763
CGATCTA23450.020.7153427
CGATCTG133750.018.8103077
TTCCGAT171950.017.8356634
ATCTAGG12200.017.8287039
GATCTGG126500.017.0799228
CACACGT332100.015.5744510-14
CCTAGAT20950.014.8811164
Produced by FastQC (version 0.11.5)