FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11904415
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC905890.7609697746592337Illumina Single End PCR Primer 1 (96% over 33bp)
GGGATCAAGCGACTAAGTGTATGTGGTGGATGCCTTGGCGATTACAGGCG196120.164745600686804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA383400.061.6711162
AGAGCGT380300.061.5241629
AGATCGG390350.060.4672161
TCGGAAG399900.058.6171954
AAGAGCG419850.055.918188
GAAGAGC449850.052.398467
GGGGGTC63200.052.3364641
ATCGGAA453750.051.742663
GGGGACG151400.046.1857261
GGGGGCC158050.045.7110751
GGGGGCT90600.043.954241
GCGACTA80100.042.4271979
GGGGCGA152650.042.0060731
GGGGACT88700.041.7882231
CGGAAGA575800.041.4524735
GGGGGAC215250.040.371071
GGGGATC136200.039.6234661
GGGGGGC147750.039.3243561
GGGATCA139000.036.6335451
GGGGACC143200.035.9643251
Produced by FastQC (version 0.11.5)