FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14909373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG1372100.9202935629821589TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGT335940.22532134651135227TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA640350.064.6988752
AGATCGG648550.064.050471
AGAGCAC634500.064.012739
TCGGAAG685600.059.8464474
GAAGAGC690800.058.8911637
GGAAGAG721400.056.7069936
ATCGGAA729300.056.528283
AAGAGCA729650.055.9536938
CGGAAGA737500.055.4887165
TCCGATC156600.054.9589775
CCGATCT159850.053.2972646
CGATCTG147850.046.1946647
GATCTGG128650.043.9021228
CTTCCGA206000.042.376433
TTCCGAT254650.034.1670154
CGATCTA51350.033.181017
GATCTAG42500.032.4132468
TCTTCCG309650.029.2927442
CTCTTCC329650.027.9562841
ATCTGGG168750.025.6498879
Produced by FastQC (version 0.11.5)