FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14909373
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC1439240.9653256377716218Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC225650.067.955721
GGGGGCC215050.064.053361
AGAGCGT591900.061.71029
AAGAGCG643550.057.2303548
GATCGGA645100.057.060992
AGATCGG646350.056.7520561
GGGGACG224500.053.8611531
TCGGAAG684650.053.4875074
GAAGAGC696250.053.2211347
GGGGGCA148900.052.660791
GGGGGTC96500.051.7902871
GGGGCGA191200.050.4568521
ATCGGAA727550.050.3564033
GGGGAGC251900.050.2485661
GGGGGCT129650.049.569851
GGGGACT112350.048.3576431
GGGGGGA218150.047.5485421
GGGGACC192400.047.3899881
GGGGAGA293400.046.67651
CGGAAGA810050.045.8779145
Produced by FastQC (version 0.11.5)