FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209844
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC1464081.199098039254228Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGACACGTAATAGCAGATTGCACAAAAATTTCTTCCCCAACGTCCCAA727060.5954703434376393No Hit
GGGACACGTAATAGCAGATTGCACAAAAATTTCTTCCCCAACGTCCCAAA320740.26268967891809264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC131250.081.52111
GGGGACA211500.072.059791
GGGGACG108350.069.69461
AGAGCGT546550.068.4279
CACGTAA175850.068.322816
AGATCGG557900.067.495061
GACACGT178650.067.008884
GGGGGCC131950.066.630111
GATCGGA566750.066.603842
AAGAGCG565050.066.494458
GAAGAGC570500.065.910047
TCGGAAG579950.064.811354
ACACGTA187450.063.746865
CGGAAGA606600.062.214585
GGAAGAG626500.060.3309366
ATCGGAA630900.059.5721933
GGGGGCT66650.058.8808941
GGGGGTC60400.057.6476551
GGGGACT67300.056.9111
ACGTAAT217750.055.4421927
Produced by FastQC (version 0.11.5)