FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11931828
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA1549371.2985185505523547TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA459400.075.5670552
AGATCGG463550.075.204611
TCCGATC78750.072.920465
AGAGCAC493650.068.985249
TCGGAAG499150.068.8511354
CCGATCT84350.067.735726
GAAGAGC515950.066.229847
CGGAAGA518850.066.19675
ATCGGAA522400.066.0780263
GGAAGAG532600.064.365466
AAGAGCA615900.055.598668
CGATCTG89150.051.1555987
CTTCCGA117250.050.0262223
GATCTGG77450.049.2403268
ATCTGGG69250.042.8211489
TTCCGAT151700.038.9047554
TCTTCCG168800.036.209662
CGATCTA34950.031.9475387
CTCTTCC219950.028.3498731
GATCTAG41000.021.0435858
Produced by FastQC (version 0.11.5)