FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14845577
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA3115062.098308472617804TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTG156960.10572846040271794Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA767050.081.827382
AGAGCAC758800.081.2160959
AGATCGG784600.080.163391
GAAGAGC792350.078.080487
GGAAGAG798300.077.634766
TCGGAAG801800.077.4128954
AAGAGCA832500.074.262298
CGGAAGA862700.072.0329065
ATCGGAA869350.071.8721853
GATCTAG17800.040.3247348
TCCGATC172250.031.5269435
CCGATCT182850.029.9767576
CTTCCGA192950.028.7827973
CGATCTA36150.025.4714247
CTAGATC47600.025.1325325
CCTAGAT35150.022.4830934
ATCTAGG18750.022.427319
TTCCGAT265750.020.8164254
TAGATCG78650.020.0962756
CGATCTG226900.019.9392077
Produced by FastQC (version 0.11.5)